Chromatography Retention Factor Calculator

Calculate Chromatographic Parameters

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Rf Value Calculator

Calculate retention factor for TLC analysis

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Resolution Calculator

Calculate resolution between two peaks

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Capacity Factor Calculator

Calculate capacity factor (k') for column chromatography

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Understanding Chromatography

Basic Concepts

Key principles:

  • Retention factor (Rf)
  • Mobile phase
  • Stationary phase
  • Elution

Parameters

Important factors:

  • Resolution (Rs)
  • Capacity factor (k')
  • Selectivity (α)
  • Plate number (N)

Techniques

Common methods:

  • TLC
  • Column chromatography
  • HPLC
  • Gas chromatography

Applications

Uses in chemistry:

  • Mixture separation
  • Compound identification
  • Purity analysis
  • Method development

Advanced Topics

Special cases:

  • Gradient elution
  • Peak tailing
  • Band broadening
  • Method optimization

Essential Chromatography Formulas

Retention Factor

Rf = distance spot/distance solvent

Resolution

Rs = 2(tR₂ - tR₁)/(w₁ + w₂)

Capacity Factor

k' = (tR - t₀)/t₀