Peak Integration
Key concepts include:
- Area calculation methods
- Baseline determination
- Peak shape analysis
- Integration parameters
- Signal-to-noise ratio
Precise Chromatographic Analysis
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Calculate peak area and concentration using internal/external standard method.
Calculate resolution between two adjacent peaks.
Calculate column efficiency using retention time and peak width.
Key concepts include:
Important parameters:
Factors affecting efficiency:
Optimization strategies:
Area ≈ 1.064 × h × w₁/₂
Where:
h = Peak height
w₁/₂ = Width at half height
Rs = 2(tR₂ - tR₁)/(w₁ + w₂)
Where:
tR = Retention time
w = Peak width
N = 16(tR/w)²
Where:
N = Number of plates
tR = Retention time
w = Peak width at base