What is Internal Standard Method?
The internal standard method involves:
- Adding known amount of reference compound
- Compensating for sample losses
- Correcting for matrix effects
- Improving precision
Calculate Concentrations Using Internal Standards
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Calculate the relative response factor using calibration standards.
Calculate unknown concentration using internal standard method.
Generate calibration curve using multiple standards.
The internal standard method involves:
Ideal internal standard should have:
For accurate results:
Used in various techniques:
RF = (Aa × Cis) / (Ais × Ca)
Where:
Aa = Analyte signal
Cis = IS concentration
Ais = IS signal
Ca = Analyte concentration
Cu = (Au × Cis) / (Ais × RF)
Where:
Cu = Unknown concentration
Au = Unknown signal
Cis = IS concentration
Ais = IS signal
RR = (Aa/Ca) / (Ais/Cis)
Linear range check:
RSD of RF < 2%